Figure 5 | Scientific Reports

Figure 5

From: High temperatures affect the hypersensitive reaction, disease resistance and gene expression induced by a novel harpin HpaG-Xcm

Figure 5

Disease resistance of tobacco to TMV that has been pretreated with purified HpaXcm, purified HpaXcm-B or purified Hpa1Xoo. (a) Tobacco leaves were pretreated with 10 μM purified HpaXcm, purified HpaXcm-B or purified Hpa1Xoo 16 h before inoculation with TMV. PBS was used as a negative control. The scale bar represents 1 cm. (b) Ratio of lesion area to tobacco leaf area. Tobacco leaves were inoculated with proteins at a concentration of 10 μM. PBS was used as a negative control. Necrotic lesions were observed 6 days after TMV inoculation. Error bars indicate the standard error of the mean (n = 3). Asterisks indicate a significant difference obtained by one-way ANOVA and multiple comparisons (LSD method, *P < 0.05, **P < 0.01). The experiment was repeated three times with the same result.

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