Figure 7 | Scientific Reports

Figure 7

From: High temperatures affect the hypersensitive reaction, disease resistance and gene expression induced by a novel harpin HpaG-Xcm

Figure 7

Expression levels of NtEXP6, Hin1, HSR203J, NPR1 and PR-1a in tobacco leaves elicited by HpaXcm at different times (0 h, 1 h, 3 h and 6 h) after heat treatment at different temperatures (28 °C, 100 °C, 150 °C and 200 °C). Relative gene expression was calculated using the 2−ΔΔCT formula with EF-1a as a control for normalization. The tobacco leaves were harvested individually at 0, 1, 3 and 6 h post spraying and frozen in liquid nitrogen. cDNA was reverse-transcribed from total RNA and subjected to a quantitative reverse-transcriptase polymerase chain reaction. Expression levels of NtEXP6, Hin1, HSR203J, NPR1 and PR-1a were quantified using a standard curve of pooled cDNA samples. Error bars indicate the standard error of the mean (n = 3). Asterisks indicate a significant difference obtained by one-way ANOVA and multiple comparisons (LSD method, *P < 0.05, **P < 0.01). The experiment was repeated three times with the same result.

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