Figure 6
From: Multilayer fabrication of unobtrusive poly(dimethylsiloxane) nanobrush for tunable cell adhesion
Control of cell adhesion using stacked layers of PDMS nanobrush. (a) Thicknesses of multiple PDMS nanobrush layers. (b) Water contact angles with increasing numbers of PDMS nanobrush layers. All the experiments were performed on at least three different samples and error bars represent the standard deviation (nmin = 3). (c) Phase-contrast images and quantification of adhered cells on varying layers of the PDMS nanobrush surfaces after 18 hours of cell seeding (scale bar = 100 μm). The percentage amount of strong adhesive states of cells in spreading shape was normalized to the total cell numbers (bottom right panel). To evaluate the ratio of strongly adhered cells, more than 100 cells for each substrate were analyzed to the total number of adhered cells. The histogram shows the means from three different image data; error bars represent the standard deviation, SD (n = 3).
