Figure 1 | Scientific Reports

Figure 1

From: Methylglyoxal down-regulates the expression of cell cycle associated genes and activates the p53 pathway in human umbilical vein endothelial cells

Figure 1

Toxicity of methylglyoxal. HUVECs were exposed to varying concentrations of methylglyoxal (MGO, 0 µM, 800 µM, 1600 µM and 3200 µM) for 24 hours. Changes in cell morphology and toxicity were assessed by phase-contrast microscopy and FACS analysis, respectively. (A) Changes in cell morphology (cell detachment, deformation and shrinkage) were apparent at 1600 µM of MGO, while at 800 µM of MGO no changes were observed. (B) Dead cells were quantitated by FACS analysis using 7-AAD-PerCP-Cy5.5. While at 0 and 800 µM of MGO the proportion of 7-ADD positive cells was comparable, at 1600 µM of MGO the percentage of 7-AAD positive cells was approximately fourfold higher, i.e. 43.9%. 7-AAD: 7-aminoactinomycin D; MGO: methylglyoxal; PerCP-Cy5.5: peridinin chlorophyll protein cyanine 5.5.

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