Figure 1
Assessment of promoter activity in L. biflexa. (A) L. interrogans lipL32 gene CDS, 5′UTR plus transcription terminator were genetically fused to different promoters. L. biflexa cell were electroporated with the different recombinant plasmids and promoter activity was indirectly accessed by probing whole cell extracts with anti-LipL32 antiserum for detecting heterologous LipL32 expression (B). Anti-DnaK antiserum was employed as a loading control. LipL32 expression was assayed in both E. coli (E) and L. biflexa (L). As a negative control, a construction in which no promoter was added to rule out the possibility of spurious DNA transcription. The E. coli and L. biflexa protein blottings are from different gels (individually depicted in Fig. S1). For comparative purposes, the lanes were cut and organized by promoters.
