Figure 4

Localization of sulfatases in central airway fibroblasts. The sulfatases (ARSB, GALNS, GNS, IDS and SGSH) were examined for localization within cultured lung fibroblasts from ever smokers and COPD patients. Panels shown are representative images of at least three ever smokers and at least three COPD patients. Sulfatases are represented in green. Golgi (stained for Golgin97, left panels) or lysosomes (stained for LAMP1, right panels) are shown in red. Nuclei are shown in blue (DAPI). Yellow indicates co-localization of sulfatase and compartment. Scale bar equals 20 μm.