Table 3 Strains used in this study.

From: Overexpression of thermostable meso-diaminopimelate dehydrogenase to redirect diaminopimelate pathway for increasing L-lysine production in Escherichia coli

E. coli strains

Relevant characteristic(s)

Reference

BL21 (DE3)

F− ompT gal dcm lon hsdSB (rB mB) λ(DE3)

Stratagene

LATR11

L-lysine producer E. coli AEChr Thr− Rifr, derived from E. coli MG1655

3

LATR12

L-lysine producer E. coli AEChr Thr – Rifr MFr, derived from E. coli LATR11

Our Lab

∆dapD/∆dapE

Knockout the natural dapD and dapE gene in strain E. coli MG1655 chromosome

Our Lab

BL21 pET28a/ddhCg

E. coli BL21 harboring the plasmid pET28a/ddhCg

This work

BL21 pET28a/ddhBf

E. coli BL21 harboring the plasmid pET28a/ddhBf

This work

BL21 pET28a/ddhCt

E. coli BL21 harboring the plasmid pET28a/ddhCt

This work

BL21 pET28a/ddhBs

E. coli BL21 harboring the plasmid pET28a/ddhBs

This work

BL21 pET28a/ddhSt

E. coli BL21 harboring the plasmid pET28a/ddhSt

This work

BL21 pET28a/ddhUt

E. coli BL21 harboring the plasmid pET28a/ddhUt

This work

LATR12∆dapD (or LATR12-1)

Knockout the natural dapD gene in strain LATR12 chromosome

This work

LATR12-1(ddhCg)

E. coli LATR12 ∆dapD harboring the plasmid pDXW-8/ddhCg

This work

LATR12-1(ddhBf)

E. coli LATR12 ∆dapD harboring the plasmid pDXW-8/ddhBf

This work

LATR12-1(ddhCt)

E. coli LATR12 ∆dapD harboring the plasmid pDXW-8/ddhCt

This work

LATR12-1(ddhBs)

E. coli LATR12 ∆dapD harboring the plasmid pDXW-8/ddhBs

This work

LATR12-1(ddhSt)

E. coli LATR12 ∆dapD harboring the plasmid pDXW-8/ddhSt

This work

LATR12-1(ddhUt)

E. coli LATR12 ∆dapD harboring the plasmid pDXW-8/ddhUt

This work

LATR12dapDA1G (or LATR12-2)

Replacement of the start code ATG by GTG in the dapD gene of strain LATR12 chromosome

This work

LATR12∆dapD::ddhSt

Replacement of the natural dapD gene with the ddhSt cassette in strain LATR12 chromosome

This work

LATR12∆rpiB::ddhSt

Replacement of the natural rpiB gene with the ddhSt cassette in strain LATR12 chromosome

This work

LATR12-2∆rpiB::ddhSt

Replacement of the natural rpiB gene with the ddhSt cassette in strain LATR12-2 chromosome

This work

LATR12-2∆rpiB::ddhCg

Replacement of the natural rpiB gene with the ddhCg cassette in strain LATR12-2 chromosome

This work

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