Figure 2 | Scientific Reports

Figure 2

From: A mutation in the AdhE alcohol dehydrogenase of Clostridium thermocellum increases tolerance to several primary alcohols, including isobutanol, n-butanol and ethanol

Figure 2

Improved n-butanol tolerance of C. thermocellum mutants. Growth rate was measured in MTC-5 medium at 55 °C. Growth comparison of wild type and butanol-tolerant mutant in different concentrations of n-butanol. Cells were grown in a 96-well plate and absorbance at 600 nm was measured at 3-minute intervals. Of the 12 isolates selected, all showed similar improvements in n-butanol tolerance, and the results from a single representative isolate are presented. The OD600 values are plotted on a semi-logarithmic scale. The orange line represents the OD600 value of the mutant strain and the light blue line represents the OD600 value of wild type strain. The wild type strain did not grow at n-butanol concentrations above 5 g/L.

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