Figure 3 | Scientific Reports

Figure 3

From: A mutation in the AdhE alcohol dehydrogenase of Clostridium thermocellum increases tolerance to several primary alcohols, including isobutanol, n-butanol and ethanol

Figure 3

Tolerance of the reconstructed C. thermocellum mutant to ethanol, n-butanol and isobutanol. Growth rate was measured in MTC-5 medium at 55 °C. Maximum specific growth rate is plotted against the concentration of the alcohol. The data represents the average of three biological replications. Error bars represent one standard deviation. Strains colored in shades of blue are wild type for the adhE gene. Strains colored in shades of red have mutations or deletion in the adhE gene. The dark blue circle represents the wild type strain LL1004; the light blue triangle (up-direction) represents the control strain, LL1160; The dark red diamond represents the adhE deletion strain, LL1111; the red square represents the selected n-butanol tolerant strain, LL1600; the light red triangle (down-direction) represents the strain with the mutant adhE (D494G mutation, strain LL1161).

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