Figure 5 | Scientific Reports

Figure 5

From: Apolipoprotein A-I enhances insulin-dependent and insulin-independent glucose uptake by skeletal muscle

Figure 5

ApoA-I increases glucose uptake in HSKMCs in an ABCA1- and SR-B1-dependent manner. HSKMCs were transfected with scrambled siRNA (scr siRNA), ABCA1 siRNA or SR-B1 siRNA. The cells were lysed and ABCA1 (A) and SR-B1 (B) protein levels were quantified by immunoblotting. Glucose uptake was measured in the ABCA1 siRNA-transfected (C) and SR-B1 siRNA-transfected cells (D) (closed bars) as described in the legend to Fig. 1. The cholesterol content of HSKMCs transfected with ABCA1 siRNA and SR-B1 siRNA was quantified as described in Materials and Methods (E). Values represent the mean ± SD of three independent experiments. Glucose uptake was normalized to 1 nmol/mg/h for control, scr siRNA-transfected cells (open bars). ***p < 0.001 vs. cells transfected with control scr-siRNA; ††p < 0.01 vs. cells transfected with ABCA1 siRNA and SR-B1 siRNA then incubated under control conditions.

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