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Figure 2

From: Critical Functionality Effects from Storage Temperature on Human Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelium Cell Suspensions

Figure 2

Temperature and Time Course Preservation of hiPSC-RPE Cell Suspensions. hiPSC-RPE cell suspension tubes were stored for 6, 24, 72, and 120 hours at 4, 16, 25, or 37 °C. (a) Trypan blue exclusion assay images of samples from each time point and temperature. Scale bars = 50 µm. (b) Quantitative analysis of viable cells in each temperature condition, grouped by time point (n = 12; mean ± SEM; *p < 0.05 and **p < 0.01 compared to all other temperatures; one-way ANOVA with Tukey’s post hoc pair-wise comparisons test). See also Supplementary Table S2.

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