Figure 6
Hypoxia Detection and Amelioration Among Varied Cell Preservation Conditions. (a) Pigmented hiPSC-RPE cells settled as sediment deposit in tube condition after 24 hours preservation at 37 °C. (b) Imaging cell hypoxia marker (green, MAR) in hiPSC-RPE cells from floating, static tube, and rotating tube preservation conditions after 6 hours in 37 °C preservation. Scale bars = 50 μm. (c) MAR fluorescence intensity quantification of samples from (b). (d) Viable cell and total cell counts of hiPSC-RPE cells from static tube and rotating tube preservation conditions after 24 hours in 37 °C preservation. (e) Cell viability frequency of hiPSC-RPE cells from static tube (control), with added Prothymosin α-derived peptide (P6Q), or with added P6Q and rotation after 24 hours in 37 °C preservation. (f) hiPSC-RPE cell suspensions of tube condition with hypoxia marker (green, MAR) at each temperature after 6 hours preservation. Scale bars = 50 μm. (g) Quantified MAR fluorescence intensity of (f) samples. n = 6. Mean ± SEM are presented. P values were calculated from one-way ANOVA with Tukey’s post hoc pair-wise comparisons test (**p < 0.01 compared to all other conditions; c,e,g) or a Student’s t test (**p < 0.01; d).
