Figure 4
From: Activator of G-protein signaling 8 is involved in VEGF-induced choroidal neovascularization
Upregulation of AGS8 mRNA expression in choroidal EC in a laser-induced CNV experiment. (A) Picture of the flat-mounted choroid 2 days post-laser-induced CNV. Mouse retinas in each eye were subjected to laser photocoagulation for induction of experimental CNV with an argon laser photocoagulator. Dissected mouse RPE-choroid was immunostained with CD31 (red; Cy3) and AGS8 (green; Alexa488) antibodies. The arrow indicates CD31-positive cells in the laser-induced CNV area, whereas the arrowhead indicates CD31-positive cells outside of the laser-induced lesions. (B) AGS8 mRNA expression in CNV area. Two days after laser-induced CNV, mouse eyes were dissected, RPE-choroid was isolated, the lesion area was dissected, and total RNA was purified. Real-time PCR was performed to evaluate laser-induced AGS8 mRNA expression. Data are means ± s.e.m from four time-independent experiments. *P < 0.05 (unpaired t-test).
