Figure 2
T cell responses after i.m. or i.d. DNA immunization. (A) IFNγ ELISPOT on splenocytes harvested from BALB/c mice 2 weeks after a single i.m. or i.d. immunization with plasmids encoding Xcl1-HA or Ccl3-HA. Splenocytes were stimulated with 2 μg/ml (left graph) IYSTVASSL (MHC-I restricted) or (right graph) HNTNGVTAACSHEG (MHC-II restricted) peptides. (B) In vivo cytotoxicity of BALB/c splenocytes pulsed with IYSTVASSL (CTVhigh) or a control peptide (DSSLQDGEFI) (CTVlow) before i.v. injection into BALB/c mice immunized two weeks prior with Xcl1-HA or CCL3-HA by i.m. or i.d. immunization. Representative histograms after i.m. DNA immunization are dispayed on the left. Percentage of CTVlow and CTVhigh cells are indicated within each histogram. The cytotoxicity data is summarized in the right graph. (C) Cytotoxicity assay as in (B) performed in BATF3 knockout mice i.m. immunized with Xcl1-HA or Ccl3-HA. (A) pooled from 3 independent experiments with 12–13 mice pr group, (B) pooled from 2 independent experiments with n = 10 mice pr group, and (C) data from one experiment with n = 4 mice pr group. Statistical analysis performed using non-parametric one-way ANOVA with Dunn’s multiple comparison test, *p < 0.05, **p < 0.01, ***p < 0.001.
