Figure 4
Xcl1-HA induces superior protection against a high dose of influenza virus after i.d. DNA immunization. (A) BALB/c mice were challenged with 5xLD50 influenza A virus (PR8) 2 weeks after a single i.d. DNA immunization with Ccl3-HA or Xcl1-HA. Weight loss was monitored over time as a sign of disease progression. (B) Survival plot of the mice presented in A. (C) BALB/c mice were vaccinated once by i.m. DNA immunization with 50 μg plasmid encoding Xcl1-HA or Ccl3-HA and subsequently challenged with 50xLD50 influenza A virus (PR8) after 2 weeks. (D) Survival plot of mice presented in C. (E) BALB/c mice were immunized by i.d. DNA immunization with 25 μg plasmid encoding Xcl1-HA or Ccl3-HA and challenged with 50xLD50 PR8 after 2 weeks. (F) Survival plot of the mice presented in E. Data shown are from one single experiment with 6 mice pr group (A and B), or pooled from 2 independent experiments with 11–12 mice per group (C–F). Statistic analysis was performed using 2-way-anova (A,C and E), or Mantel Cox (B,D and F), *p < 0.05, ***p < 0.001.
