Figure 4 | Scientific Reports

Figure 4

From: Notch signaling facilitates hepatitis B virus covalently closed circular DNA transcription via cAMP response element-binding protein with E3 ubiquitin ligase-modulation

Figure 4

Notch signaling affects cAMP response element-binding protein (CREB) 1 and CREB-binding protein (CBP) gene expression. (A) RTD-PCR analysis of CREB1 and CBP gene levels after Notch inhibition by Jagged1 shRNA transfection. (B) Protein expression levels were confirmed by western blotting using anti-CREB, anti-phospho-CREB (Ser133), and anti-CBP antibodies after Jagged1 shRNA transfection. Relative pixel intensities (right) were normalized to the GAPDH pixel intensity (Control-shRNA = 1). (C) CREB1 and CBP gene expression levels after DAPT treatment. (D) Protein expression of CREB, phospho-CREB (Ser133), and CBP after DAPT treatment. Relative pixel intensities (right) were normalized to the GAPDH pixel intensity (DMSO = 1). (E) RTD-PCR analysis of CREB1 and CBP gene expression levels after MG-132 treatment. (F) CREB, phospho-CREB (Ser133), and CBP protein expression by western blotting after MG-132 treatment. Relative pixel intensities (right) were normalized to the GAPDH pixel intensity (DMSO = 1). Quantitative gene expression data represent the mean ± standard deviation of three independent experiments and were normalized to the expression levels of human GAPDH. *P < 0.05 and **P < 0.01 versus the corresponding control shRNA and DMSO (Control-shRNA, DMSO = 1).

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