Table 2 Results of three way ANOVAs for pHECM, pHi (Fig. 1) and calcification, and two way ANOVAs for photosynthesis and respiration (Fig. 2).

From: Effects of light and darkness on pH regulation in three coral species exposed to seawater acidification

Variable

Effect

df

MS

F

P

Post hoc

pHECM

pHSW

3.00

1.56

242.66

<0.0001

8.1 > 7.8, 7.4, 7.2; 7.8 > 7.4, 7.2; 7.4 > 7.2

 

L/D

1.00

0.55

85.48

<0.0001

Light > Dark

 

Sp

2.00

0.25

38.33

<0.0001

SP > PD, AH

 

pHSW * lightdark

3.00

0.02

2.70

0.05

 
 

pHSW * species

6.00

0.02

2.48

0.03

See S 1

 

lightdark * species

2.00

0.09

13.70

<0.0001

See S 1

 

pHSW * lightdark * species

6.00

0.02

3.34

0.01

See S 1

 

Error

96.00

0.01

   

pHI

pHSW

3

0.22

107.86

<0.0001

8.1 > 7.8, 7.4, 7.2; 7.8 > 7.4, 7.2; 7.4 > 7.2

 

L/D

1

0.04

18.79

<0.0001

Light > Dark

 

Sp

2

0.55

271.63

<0.0001

SP > PD; SP > AH; PD > AH

 

pHSW * lightdark

3

0.00

1.27

0.294

 
 

pHSW * species

6

0.01

4.94

<0.001

See S 1

 

lightdark * species

2

0.00

1.19

0.314

 
 

pHSW * lightdark * species

6

0.00

0.25

0.955

 
 

Error

48

0.00

   

Calcification rate

pHSW

3

8394.50

45.93

<0.0001

8.1 > 7.4, 7.2; 7.8 > 7.4, 7.2; 7.4 > 7.2; 8.1 = 7.8

 

Light/Dark

1

57911.62

316.87

<0.0001

Light > Dark

 

Species

2

16810.57

91.98

<0.0001

PD > SP; PD > AH; SP > AH

 

pHSW * lightdark

3

297.56

1.63

0.195

 
 

pHSW * species

6

899.73

4.92

<0.001

See S 1

 

lightdark * species

2

1803.86

9.87

<0.0001

See S 1

 

pHSW * lightdark * species

6

831.49

4.55

<0.001

See S 1

 

Error

48

182.76

   

Photosynthetic rate

pHSW

3

0.16

0.24

0.87

 
 

Species

2

7.49

11.22

<0.0001

AH > SP, PD

 

pHSW * Species

6

2.67

3.99

<0.0001

See results text

 

Error

48

0.67

   
 

Total

     

Respiration rate

pHSW

3

0.37016

0.649083

0.587392441

 
 

Species

2

6.42856

11.272649

<0.0001

SP > AH, PD

 

pHSW * Species

6

0.38002

0.6663842

0.677041204

 
 

Error

48

0.57028

   
  1. SP = Stylophora pistillata; PD = Pocillopora damicornis; AH = Acropora hyacinthus.