Figure 3
From: The AAA+ protease ClpXP can easily degrade a 31 and a 52-knotted protein
Models of ClpXP-catalysed degradation for trefoil knotted proteins. Trefoil knotted protein (orange), ssrA-tag (red), ATP-dependent protease (blue/red), highly stable ThiS ‘plug’ domain (green). (A) Knot tightening and translocation into the degradation machinery. (B) Knot slips off the end of the polypeptide chain as the protein is unfolded and translocated into ClpXP. (C) Knot slipping along the chain before tightening and being blocked by the addition of a highly stable ThiS domain.
