Figure 2 | Scientific Reports

Figure 2

From: Transcriptome-based identification and validation of reference genes for plant-bacteria interaction studies using Nicotiana benthamiana

Figure 2

geNorm analysis of selected reference genes in N. benthamiana leaves infiltrated with different Pseudomonas strains. (A) N. benthamiana reference genes were ranked based on expression stability calculated by geNorm. M values represent the average pairwise variation of the gene compared with all other control genes. (B) Pairwise variation (Vn/Vn + 1) for determination of the optimal number of reference genes. The pairwise variation was calculated considering all the samples treatments and time-points together (Total), mock and P. fluorescens (PTI), Pst DC3000 and Pst DC3000 ΔhopQ1-1 (ETI), samples taken at 6 hai (6 h) or samples taken at 12 hai (12 h).

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