Figure 4 | Scientific Reports

Figure 4

From: Transcriptome-based identification and validation of reference genes for plant-bacteria interaction studies using Nicotiana benthamiana

Figure 4

Relative expression of NbNAC042 analyzed using different reference genes. Relative expression analysis by RT-qPCR at two time points (6 and 12 hai) using plants infiltrated with: (A), mock (10 mM MgCl2) or 108 cfu/ml Pseudomonas fluorescens 55 (Pf); (B), 5 × 106 cfu/ml of Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) or Pst DC3000 ΔhopQ1-1 (ΔhopQ1-1) strains. In both cases, the geometric mean of the two best (NbUbe35/NbNQO), the intermediate (NbTspan) or the worst (NbEF1α) reference genes was used for normalization of the data. The relative expression was expressed as E−ΔΔCq, where E corresponds to the primer efficiency value. Calibration samples are 10 mM MgCl2 6 h in A and Pst DC3000 6 h in B (biological replicate 1 in both cases). Bars represent the mean of three biological replicates and three technical replicates with their corresponding standard deviation. ** or * indicate significant differences using Student t-test with p-values < 0.01 or <0.05, respectively.

Back to article page