Table 1 Summary of the experiments performed.

From: Transcriptome-based identification and validation of reference genes for plant-bacteria interaction studies using Nicotiana benthamiana

Plant

Inoculum

Concentration

Immune response evaluated

Time points

Experiment

Nicotiana benthamiana

MgCl2

10 mM

PTI

6 h

RNA-seq

Pseudomonas fluorescens 55

108 cfu/ml

MgCl2

10 mM

PTI

6, 12 h

Validation of RT-qPCR reference genes

Pseudomonas fluorescens 55

108 cfu/ml

Pst DC3000a

5 × 106 cfu/ml

ETI

Pst DC3000 ΔhopQ1-1b

  1. aPseudomonas syringae pv. tomato (Pst) DC3000.
  2. bPseudomonas syringae pv. tomato (Pst) DC3000 mutant, lacking HopQ1-1 effector.
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