Table 2 Analysis of ten selected N. benthamiana reference genes using Bestkeeper algorithm.

From: Transcriptome-based identification and validation of reference genes for plant-bacteria interaction studies using Nicotiana benthamiana

Ranking

1

2

3

4

5

6

7

8

9

10

Gene name

NbTspan

NbUbe35

NbNQO

NbErpA

NbPP2a

NbCENPO

NbPGK

NbKLC

NbGADPH

NbEF1α

Geo Mean [Cq]

25.02

26.75

28.46

28.58

27.02

29.45

25.57

29.69

24.31

17.86

Min [Cq]

24.37

25.93

27.54

27.41

25.83

28.21

24.14

27.72

22.81

15.24

Max [Cq]

25.80

27.83

29.51

29.80

28.20

30.80

27.58

32.27

26.65

20.44

SD [ ± Cq]

0.28

0.39

0.41

0.42

0.45

0.53

0.53

0.69

0.70

0.74

CV [% Cq]

1.13

1.44

1.46

1.47

1.67

1.81

2.05

2.34

2.87

4.12

Min [x-fold]

−1.57

−1.71

−1.83

−2.14

−2.25

−2.33

−2.69

−3.65

−2.79

−5.61

Max [x-fold]

1.71

2.03

1.98

2.23

2.24

2.49

4.03

5.48

5.03

5.45

SD [ ± x-fold]

1.21

1.30

1.33

1.34

1.37

1.44

1.44

1.61

1.62

1.66

Coeff. of corr. [r]

0.33

0.70

0.69

0.78

0.88

0.58

0.84

0.76

0.78

0.81

p-value

0.004

0.001

0.001

0.001

0.001

0.001

0.001

0.001

0.001

0.001

  1. [Cq], quantification cycle; Geo Mean [Cq], geometric mean of Cq; Min and Max [Cq], the extreme values of Cq; SD [Cq], standard deviation of Cq; CV [%Cq], coefficient of variance expressed as a percentage on the Cq level; Min [x-fold] and Max [x-fold], the extreme values of expression levels expressed as an absolute x-fold over or under regulation coefficient; SD [±x-fold], standard deviation of the absolute regulation coefficients, Coeff. of corr [r], coefficient of correlation between each candidate and the BestKeeper index.
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