Figure 1
From: Molecular layer interneurons shape the spike activity of cerebellar Purkinje cells
The Ascl1CreER allele can be used for genetic marking of stellate cells and basket cells. (a) Schematic of cerebellar circuitry. The Purkinje cell (PC), basket cell (BC), and stellate cell (SC) are colorized while other cells and fibers in the cerebellar cortex are represented in grayscale (climbing fiber = CF, mossy fiber = MF, Golgi cell = GoC, granule cell = GrC, unipolar brush cell = UBC). Dotted lines represent the borders of the Purkinje cell layer (PCL) with the molecular layer (ML) and granule cell layer (GL). (b,c) Golgi-Cox stain of cerebellar tissue. Scale = 50 μm. (b) Basket cell (arrowhead) and Purkinje cell (asterisk) revealed by Golgi-Cox stain. (c) Stellate cell (arrowhead) and Purkinje cells (asterisks) revealed by Golgi-Cox stain. (d) Representation of breeding scheme. (e) Schematic of methods for tamoxifen administration. Tamoxifen was administered via oral gavage to pregnant dams at E18.5 to achieve constitutive marking and manipulation of a subset of basket cells in the resulting pups (upper left). Tamoxifen was administered via subcutaneous injection into the scruff of pups at P4 to achieve constitutive marking and manipulation of a subset of stellate cells (bottom right). (f) Labeled cells were found in the basal molecular layer in animals treated with tamoxifen at the basket cell timepoint and the apical molecular layer for those treated at the stellate cell timepoint (g). Scale = 50 μm. 5 sections separated by ~200 µm around midline per mouse, N = 7 for each condition.
