Figure 6

Conditional deletion of Vgat with Ascl1^CreER occurs in extracerebellar cell types that are unlikely to affect Purkinje cell activity in this manipulation. (a) Sparse labeling of cells occurs outside the cerebellum at the basket cell time point. Scale = 1 mm. (b–g) Many of the cells outside the cerebellum had morphologies that resembled glia, with the notable exception of cells in the olfactory bulb (g) where the majority of cells had the morphology of neurons, though cells with glial-like morphology were also present. Scale = 20 μm. (h) Sparse labeling outside of the cerebellum also occurred at the stellate cell time point. Scale = 1 mm. (i–n) While again many of the cells had morphologies that resembled glia, some cells with neuron-like morphologies were also detected. (k) Very sparse labeling of putative granule cells in the dentate gyrus occurred at the stellate cell time point, unlike at the basket cell time point at which no neurons were detected in the hippocampus. (n) Similar to the basket cell time point, many neurons in the olfactory bulb were labeled in addition to some glial-like cells. Scale = 20 μm. (o) Recombined cells with glial-like morphologies co-labeled with GFP and CAII, a maker of oligodendrocytes. Scale = 20 μm. N = 2, n ≥ 6. (p) Cells with neuron-like morphologies in the dentate gyrus of the hippocampus co-labeled with GFP and NeuN. Scale = 20 μm. N = 2, n ≥ 6. (q) Both neurons (arrow, co-labeled with GFP and NeuN) and glia (arrowhead, only labeled with GFP and not by NeuN) were labeled in the olfactory bulb. Scale = 20 μm. N = 2, n ≥ 6.