Table 2 SMM data for replication thermosensitive mutants.

From: Single molecule tracking reveals functions for RarA at replication forks but also independently from replication during DNA repair in Bacillus subtilis

Background

D (μm2 s−1)

25 °C −drug

42 °C −drug

42 °C+H2O2

42 °C+MMC

static dyna

static dyn

static dyn

static dyn

static dyn

Wild type

0.23 2.3

48 52

45 55

ND ND

ND ND

dnaB37

0.2 1.8

41 59

65 35

40 60

58 42

dnaC30

0.19 2.0

38 62

29 71

38 62

48 52

  1. Apparent diffusion coefficient (in μm2 s−1) and population weights (in %) calculated by step-size distribution and Gaussian fit for RarA-mVenus in the replication deficient thermosensitive mutants studied in exponential growth for 60 min at permissive (25 °C) and non-permissive (42 °C) temperature in the presence or absence of H2O2 (0.5 mM) or MMC (50 ng/ml). At least 200 tracks/condition were considered for the analysis. adyn, dynamic; ND, not done.
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