Figure 1 | Scientific Reports

Figure 1

From: Evaluation of site-specific homologous recombination activity of BRCA1 by direct quantitation of gene editing efficiency

Figure 1

Scheme of knock-in of a marker sequence into an endogenous target gene by gene editing. (a) Illustration of the knock-in. (b) Structure of the donor vector containing 3xFLAG sequence between the homology arms for the ACTB gene. The target sequence of the gRNA, highlighted in yellow, is placed on the anti-sense strand around the stop codon (highlighted by red color) of ACTB. The DSB is created 10 bp upstream from the stop codon and 3 bp downstream of the PAM sequence (indicated by the scissors). The donor vector contains 5′ and 3′ arms and a marker sequence, highlighted in blue, just upstream of the stop codon.

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