Figure 6
Dependence of knock-in on HR or NHEJ factors. (a) Effects of knocking down RAD51, 53BP1 or LIG4 on the expression of β-actin::3xFLAG. Transfection was performed according to Protocol 1 in Fig. 3a. (b,c) qPCR analysis of knock-in at the ACTB (b) or KLF7 (c) locus in RAD51, 53BP1, or LIG4 knock-down cells. Genomic DNA was extracted 48 h after transfection of ACTB-Cas9 and a donor vector (Fig. 3a, Protocol 1). The amount of the fusion gene was calculated relative to a sample transfected with control siRNA. Error bars indicate ± SEM of three independent experiments. Significant differences relative to the sample transfected with control siRNA are indicated by asterisks. *p < 0.05, **p < 0.01, n.s., not significant.
