Figure 4 | Scientific Reports

Figure 4

From: PRSS3/Mesotrypsin and kallikrein-related peptidase 5 are associated with poor prognosis and contribute to tumor cell invasion and growth in lung adenocarcinoma

Figure 4

KLK5 silencing inhibits invasion of PC9 lung adenocarcinoma cells. (a) KLK5 expression is effectively suppressed by two targeted lentiviral shRNA constructs, KLK5-KD1 and KLK5-KD2, relative to cells transduced with the non-target control virus (NT), as assessed by qRT/PCR normalized to GAPDH expression. (b) KLK7 expression is effectively suppressed by two targeted lentiviral shRNA constructs, KLK7-KD1 and KLK7-KD2, relative to cells transduced with the non-target control virus (NT), as assessed by qRT/PCR normalized to GAPDH expression. (c) Knockdown of KLK5 significantly inhibits PC9 cellular invasion in Matrigel transwell assays. (d) By contrast, knockdown of KLK7 has no significant impact on PC9 cellular invasion in Matrigel transwell assays. Representative images of the invasion assays are shown above each condition. Error bars show SEM. **P ≤ 0.01; ***P ≤ 0.001.

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