Figure 7

Galectin-3 silencing changes levels of MMP2 and MMP9 in HTR-8/SVneo cells (abbreviated gal-3 in the figure). The effect was studied at mRNA level for both gelatinases, (a,b) difference significant for MMP2 and MMP9, in silenced vs. lipofectamine only. The graph shows the average of ten experiments. The data didn’t pass Shapiro-Wilk normality test and non-parametric Kruskal-Wallis test was used followed by Dunn’s Multiple Range test (α = 0.05) for post-hoc comparisons. (c) A representative gelatin zymography after galectin-3 silencing; control (L), scramble-transfected (S) and galectin-3 transfected (sigal-3). Graphs give the average of eight independent experiments for MMP2 (d) and MMP9 (e); Open bars represent control (L), light gray bars scramble-transfected (S) and dark grey bars galectin-3 transfected (sigal-3). Data passed Kolmogorov-Smirnov normality test and were analysed by one-way analysis of variance (ANOVA) with Tukey post-hoc test (α = 0.05). Differences were significant at *p < 0.05, **p < 0.01, and at ***p < 0.001.