Figure 4
From: Imaging of nanoparticle dynamics in live and apoptotic cells using temporally-modulated polarization

Fixed B-16 cells, labeled by GNRs, demonstrate an enhanced resolution when imaged by PMLI in the DF mode. (a) A conventional DF imaging yields a blurry image of a fixed B16 cell, in spite of it being labeled by the GNRs. (b) The image is significantly sharpened and its SNR is improved by the DF PMLI method. The scale bar lengths (white) correspond to 10 μm. The bottom images zoom-in onto the field of view marked by a red dashed rectangle. Remarkably, we demonstrate here the capability of the PMLI to separate between two adjacent pixels (a red triangle and a blue dot), in spite of them being much closer together than the optical resolution limit. The red pixel is blinking at the modulation frequency (solid red curve in (c)), hinting it to be a true scattering from a GNR. The blue pixel does not blink at the same frequency (dashed blue curve in (c)), indicating it to be induced by the environmental noise.