Figure 5
From: Phosphate binding by sucroferric oxyhydroxide ameliorates renal injury in the remnant kidney model
Renoprotective effects of SF is associated with the alleviation of renal inflammation and fibrosis. (a) Quantitative analysis of MCP-1, osteopontin (OPN), PAI-1, TGF-β, and α1(I) collagen (Col1a1) gene expression by real time RT-PCR in the kidney of RK and RK+SF rats (n = 8 each group). (b) Immunohistochemical staining for ED-1 (CD68) in the kidneys of RK (left) and RK + SF (right) rats. Infiltration of the CD68-positive macrophages (indicated by arrows) in RK rats was ameliorated by SF. Bar represents 100 μm. (c) Masson’s trichrome staining of the kidney sections from RK (left) and RK + SF (right) rats. Fibrotic area was reduced by SF administration. Bar represents 100 μm. (d,e) Quantitative analysis of the CD68-positive area (d) and fibrosis area (e) (see also Methods; n = 4 each group). Data are expressed as mean ± SEM; *P < 0.05. **P < 0.01. ***P < 0.001.
