Figure 3
Chemogenetic activation of the LHb induces thermal hyperalgesia in Naïve rats. (A,B) Representative figures (A) and pooled data (B) showing that c-fos expression in the LHb is increased after CNO systemic application in hM3Dq overexpressed rats. Unpaired t-test, t = 4.891, **p < 0.01. Nrat = 4–5/group. (C) Bath-applied CNO (10 μM) sharply increased the firing rate of LHb neurons infected with AAV-CaMKIIa-hM3Dq-mCherry viruses. (D) After systemic CNO injection (1 mg/kg, i.p.), rats infected with hM3Dq showed a decreased paw withdrawal latency than those infected with eGFP. Two-way ANOVA, for group F2,30 = 3.583, P = 0.0402, for Time F4,120 = 4.534, P = 0.0019, post-hoc: *p < 0.05, **p < 0.01 vs GFP + CNO; #p < 0.05, ##p < 0.01 vs. hm3Dq+Veh; Nrat = 9–12/group.
