Figure 4
Intra-LHb M-channel activators reduces thermal hyperalgesia in EtOH-WD rats. (A–C) Representative expression of KCNQ2 (A), KCNQ3 (B) and pooled results (C) of Western blots show the reduced KCNQ2 and KCNQ3 expression in the LHb from ethanol-withdrawn rats compared to CTRL rats. Note an even greater loss of KCNQ3 expression in the EtOH-WD rats. Unpaired t-test, p < 0.05, ***p < 0.001. Nrat = 6/group. Full-length blots are presented in Supplementary Fig. 1. (D) Cresyl violet-stained brain sections show the accurate guide cannula placements above the LHb. Scale bar = 1.0 mm. (E, F) Intra-LHb retigabine and ICA-27243 increased the paw withdrawal latency (PWL) in EtOH-WD rats (Two-way ANOVA, for Drug F2,35 = 0.0017, P = 0.0017, for Time F4,140 = 0.6.256, P = 0.0001, post-hoc: **p < 0.01 vs. Retigabine, #p < 0.05, ###p < 0.001 vs. ICA-27243, Nrat = 19(aCSF), 12(Retigabine), 7(ICA-27243).
