Figure 3
Increased expression of p53 and p53 targets in PRMT1-KD SK-N-SH cells. (A) RNA levels of PRMT1, p53 and p53 target p21, PAI-1, GADD45 and 14-3-3-σ in non-infected, control vector-infected, and PRMT1-A1 or B1 shRNA-infected SK-N-SH cells determined by qRT-PCR. Experiments were performed at least three times. The data are shown as the mean ± SD of triplicates. * indicates p < 0.05 and ** indicates p < 0.01. (B) Western blot analyses of p53 and phosphorylated p53, p21 and PAI-1 in non-infected, control vector-infected, and PRMT1 A1 plus B1 shRNA-infected (PRMT1-KD) SK-N-SH cells. The immunoblots shown are the representative of three independent experiments.
