Figure 8
Inhibition of PRMT1 by a specific inhibitor K313 induced p21 and PAI-1 expression in SK-N-SH cells. (A) SK-N-SH cells were treated with a PRMT1 inhibitor K313 (2 μM) or not for 7 days with medium change every 3 days. Cell extracts (25 μg of protein) were immunoblotted with asymmetric di-methylarginine-specific antibodies ASYM24 (left) and ADMA (right). +/− indicate the addition of K313 or not. The immunoblots shown are representatives of independent experiments. (B) RNA levels of p53 target p21 and PAI-1 under the treatment of K313 or not. ** indicates p < 0.01.
