Figure 4 | Scientific Reports

Figure 4

From: miR-302a inhibits human HepG2 and SMMC-7721 cells proliferation and promotes apoptosis by targeting MAP3K2 and PBX3

Figure 4

siMAP3K2 or siPBX3 effects on MAPK signaling pathway. (A) siMAP3K2 was transfected into HepG2 cells and SMMC-7721 cells. Then mRNA and protein expression were detected for interference efficiency verification (**P < 0.01). (B) The key factors in MAPK pathway were detected in HepG2 cells after siMAP3K2 transfection, including p-ERK, p-JNK and p-p38. (C) The HepG2 cells and SMMC-7721 cells were cultured and transfected siPBX3 vector (**P < 0.01). (D) The p-ERK, p-JNK and p-p38 were detected by western blot in cells after PBX3 interference. Statistical analysis was conducted using Fisher’s least significant difference (LSD) method of two-way analysis of variance (ANOVA).

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