Figure 2

Adipose-specific L-PGDS KO mice. (a) Strategy to generate the aP2-Cre/L-PGDS ^flox/flox and AdipoQ-Cre/L-PGDS ^flox/flox mice. Wild-type, targeted, floxed, and null L-PGDS gene loci were shown. Primers used for genotyping by PCR were indicated as arrows. Exons were shown as boxes, and the number of exons were shown above boxes. Neo means the gene for neomycin phosphotransferase. FRT means the flippase recognition target. (b) Expression of the Cre and L-PGDS genes in the HFD-fed wild-type (W; white columns), L-PGDS ^flox/flox (C; gray columns), aP2-Cre/L-PGDS ^flox/flox and AdipoQ-Cre/L-PGDS ^flox/flox (LKO; black columns) mice. Mice (7-week-old; n = 5) were fed an HFD for 11 weeks and the mRNA levels of both genes in the brain, liver, and vWAT was measured by qPCR. n.d. means not detected. Data are shown as means ± S.D. ^#p < 0.01, as indicated by the bracket. (c) Western blot analysis of L-PGDS expression in the brain, liver, and vWAT of the HFD-fed L-PGDS ^flox/flox (C), aP2-Cre/L-PGDS ^flox/flox, and AdipoQ-Cre/L-PGDS ^flox/flox (LKO). Protein (20 μg) was applied in each lane. Data are the representative of each of the three mice.