Figure 4

Decrease of body fat in the HFD-fed aP2-Cre/L-PGDS ^flox/flox mice. (a) PGD₂ level in vWAT of the HFD-fed L-PGDS ^flox/flox (C; gray column) and aP2-Cre/L-PGDS ^flox/flox (LKO; black column) mice. Data are shown as means ± S.D. ^#p < 0.01, as indicated by the bracket. (b) CT analysis. Abdominal cross-section of the LFD- or HFD-fed control L-PGDS ^flox/flox (C) and aP2-Cre/L-PGDS ^flox/flox (LKO) mice (18-week-old). vWAT, sWAT, and internal organs were shown by pink, yellow, and light blue, respectively. (c) Quantification of fat (vWAT and sWAT) in the whole body of the LFD- or HFD-fed control L-PGDS ^flox/flox (C) and aP2-Cre/L-PGDS ^flox/flox (LKO) mice (18-week-old) by using of LaTheta software (Aloka). Fat (%) means the ratio of the weights of total fat per body weight. Data are shown as means ± S.D. *p < 0.01, as indicated by the brackets. Significant difference between aP2-Cre/L-PGDS ^flox/flox (LKO) mice and control L-PGDS ^flox/flox (C) under HFD conditions was shown as ^#p < 0.01 with the brackets. (d) Adipose size of vWAT in the 18-week-old control L-PGDS ^flox/flox (C) and aP2-Cre/L-PGDS ^flox/flox (LKO) mice under LFD or HFD conditions, stained with hematoxylin and eosin. Bar = 50 μm. (e) Quantification of adipocyte area shown in (d) was performed on hematoxylin and eosin-stained sections using ImageJ software. At least 200 adipocytes from five mice in each group were measured. Data are shown as means ± S.D. *p < 0.01, as indicated by the brackets. Significant difference between aP2-Cre/L-PGDS ^flox/flox (LKO) mice and control L-PGDS ^flox/flox (C) under HFD conditions was shown as ^#p < 0.01 with the brackets.