Figure 1 | Scientific Reports

Figure 1

From: Synchronized Orchestration of miR-99b and let-7g Positively Regulates Rotavirus Infection by Modulating Autophagy

Figure 1

RV infection upregulates miR-99b. HT29 cells were mock treated or infected with RV-SA11 for indicated time points. Total RNA from mock- or virus-infected cells was extracted. (A) Expression of miR-99b was analyzed in RV-infected HT29 cells by qRT-PCR and normalized to the expression of U6 snRNA. (B) RV-VP6 RNA level was measured in SA11 infected HT29 cell lysate by qRT-PCR and plotted as relative RNA level in comparison to mock-infected cells. (C) Relative expression of miR-99b was analyzed by qRT-PCR in UV inactivated RV-SA11 infected (8hpi) HT29 cells. (D) RV-VP6 RNA was measured by qRT-PCR in UV-inactivated RV-SA11 infected cells. Results are presented as the means and standard deviations from at least three independent experiments.

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