Figure 5 | Scientific Reports

Figure 5

From: Synchronized Orchestration of miR-99b and let-7g Positively Regulates Rotavirus Infection by Modulating Autophagy

Figure 5

miR-99b and let-7g regulate autophagy during RV infection. HT29 cells were transfected with scrambled-miR, mimic let-7g, anti-miR-99b (40 nM each) or in combination (antagonist miRNA cocktail −50 nM total) and infected with RV-SA11. (A) The expressions of TSC1, TSC2, mTOR, and p-mTOR were examined by immunoblot analysis. GAPDH was used as an internal control for obtaining the expression levels after normalization. VP6 expression was checked to ensure virus infection. (B) Expressions of LC3I/II, Beclin1 and ATG5 were checked in the same cell lysates by immunoblotting. Normalization was done by GAPDH. Virus infection was confirmed by analysing NSP3 expression in the same cell lysates. Results are presented as the means and standard deviations from three experimental replicates.

Back to article page