Table 1 Bacterial inhibitory concentration and relative bacterial inhibitory concentration values from activity measurements of GxxxG and GxxxG-like peptide variants of Pls-α and Pls-β together with the wild type complementary peptide.

From: NMR structures and mutational analysis of the two peptides constituting the bacteriocin plantaricin S

Peptide combinationb

Bacterial inhibitory concentration (nM)

Relative bacterial inhibitory concentration valuesa

Lac. lactis IL1403

Lab. sakei NCFB 2714

Lac. lactis IL1403

Lab. sakei NCFB 2714

α + β

0.6 ± 0.1

0.17 ± 0.04

1

1

α(G9A) + β

11.4 ± 0.9

3.7 ± 1.0

20

20

α(G9L) + β

>25

>25

>40

>150

α(G13A) + β

1.0 ± 0.1

0.36 ± 0.05

2

2

α(G13L) + β

>25

>25

>40

>150

α(A15G) + β

6.1 ± 1.5

1.25 ± 0.75

10

7

α(A15L) + β

0.7 ± 0.1

0.25 ± 0.04

1

1

α(G19A) + β

0.8 ± 0.2

0.22 ± 0.04

1

1

α(G19L) + β

4.3 ± 0.6

0.33 ± 0.02

7

2

β(S6G) + α

0.8 ± 0.1

0.23 ± 0.06

1

1

β(S6A) + α

0.6 ± 0.1

0.15 ± 0.05

1

1

β(S6L) + α

1.2 ± 0.1

0.29 ± 0.08

2

2

β(A10G) + α

1.7 ± 0.4

0.41 ± 0.12

2

2

β(A10L) + α

0.7 ± 0.1

0.26 ± 0.06

1

1

β(A14G) + α

3.1 ± 0.4

0.59 ± 0.09

5

3

β(A14L) + α

1.4 ± 0.1

0.36 ± 0.06

2

2

β(S17G) + α

5.4 ± 0.3

1.1 ± 0.35

9

6

β(S17A) + α

2.4 ± 0.2

0.51 ± 0.19

4

3

β(S17L) + α

10 ± 1

1.3 ± 0.9

17

8

β(G21A) + α

4.1 ± 0.3

1.0 ± 0.47

7

6

β(G21L) + α

>25

>25

>40

>150

  1. aThe relative bacterial inhibitory concentration is defined as a fold increase or decrease in activity compared to the wild type combination.
  2. bThe peptides were added in equimolar amounts. α and β are Pls-α and Pls-β, respectively. Single amino acid substitutions are indicated in parentheses.
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