Figure 5
From: Harnessing neurovascular interaction to guide axon growth
Patterned microvessels guide axons from NPCs in vitro (Ai) Day 4 axon alignment with microvessels (direction denoted by white arrow), (ii) ZO-1 tight junction stain (red), and (iii) axons labeled with Tuj (cyan) for flow condition. (B) Higher magnification images from A. (Ci) Day 4 cd44KD with flow condition, (ii) ZO-1 tight junction stain (red), and iii) Tuj-positive axons (cyan), GFP hBVP (green), DAPI (blue). (D) Higher magnification images from C. (E,F) Axon and vessel alignment quantification for flow and cd44KD conditions. (G,H) Axon length and branch number for both flow and cd44KD conditions. Scale bars, 50 μm. Data are presented as mean ± s.e.m. *P < 0.05 compared to untreated flow condition. Alignment values (n = 30), axon length values (n = 15), and branch number/axon values (n = 5) are from single hydrogel samples per condition.
