Figure 4

(A) Sgroppino localizes to peroxisomes. Schematic representation of the approach used to quantify colocalization between Sgroppino and Peroxisomal Membrane Protein (PMP34). (B) Localization of RFP-tagged Sgroppino and GFP-tagged PMP34 in Drosophila S2 cells. Expression plasmids were co-transfected with dsRNA targeting Sgp, PMP34, or, as a non-targeting control, Luciferase (dsLuc), and cells were fixed and processed two days later. Images were obtained by confocal microscopy.