Figure 5
Sgroppino-deficiency causes weight increase and accumulation of fat. (A) Time to pupation of wild-type and Sgp mutant flies. Fifty eggs were incubated on standard cornmeal-agar media at 25 °C, and monitored for the appearance of pupae at least twice a day. The 0 h time point corresponds to the appearance of the first pupae, which was identical for wild-type and mutant flies. (B) Weight of female and male wild-type and Sgp mutant flies. Three to five-day-old flies were weighed in groups of 10 on a precision scale. (C,D) Levels of triglycerides in 3 to 5-day-old female wild-type and Sgp mutant flies at steady-state (C) or upon DCV infection (D). All DCV infected Sgp mutant flies had died at 6 dpi. (E) Levels of peroxidated lipids in young (2–4 day old) or aged flies (10–12 day old). Data represent mean and s.d. of three biological replicates of (A) 50 eggs, (B) 10 flies, (C,D) 2 flies, and (E) 20 to 40 flies for each genotype. Mock infection was performed with Tris Buffer, and harvested at day 2 (D). Student’s t-tests were used to compare the differences in weight and triglycerides (**P < 0.01, ***P < 0.001).
