Table 1 Residues involved in the interaction with OLE as derived from the NMR analysis are presented in the column “NMR”.

From: Impact of lipid binding on the tertiary structure and allergenic potential of Jug r 3, the non-specific lipid transfer protein from walnut

Residues of Jug r 3

NMR

Cluster1 OLE

Ser 10

*

 

Leu 18

a

*

Leu 36

*

*

Arg 46

a

*

Ala 49

*

 

Cys 50

*

*

Leu 53

*

*

Leu 63

*

*

Ala 68

*

 

Val 79

*

*

Pro 80

b

*

Tyr 81

NA

*

Val 92

*

*

  1. Residues for which intermolecular non-bonded contacts with OLE are present in at least three of the best five model structures are reported in the column “Cluster1 OLE”. The occurrence of interaction in a cluster is indicated by an asterisk (*).
  2. aThese residues have not been reported due to spectral overlap of their backbone amides or as they have a chemical shift perturbation lower than 0.16 ppm. bProline residue has no amide proton. NANot assigned residues in free Jug r 3.
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