Table 2 SNPs and their corresponding oligonucleotide sequences.

From: Cost-effective and robust genotyping using double-mismatch allele-specific quantitative PCR

 

allele

forward

reverse

template

rs6734469

A/G

TCCCCCACTGATTTCTTCTGCAC[A/G]

ACTGTCAGCACCAGAGGGAC

TCCCCCACTGATTTCTTCTGTAC[A/G]AGATACTCTACTCTGGGTCCCTCTGGTGCTGACAGT

rs187115

C/T

CAGCGAGATAGTTGTGCTGACA

GAGTGAGAAAGATGTTATCATCGTCACTG[A/G]

CAGCGAGATAGTTGTGCTGACAGTCATATA[C/T]CAATGACGATGATAACATCTTTCTCACTC

rs1027154

C/G

CTCCATACACTTTCTTAGGTGACTCTT[C/G]

ACCAGAGCATTCACCCTCAG

CTCCATACACTTTCTTAGGTGACTTTT[C/G]CGAAGTGAACCACTGAGGGTGAATGCTCTGGT

rs2168945

A/C

GGAATAGGAAATGTGATGCAAGCCGA[A/C]

TTGAATAGGGCAACGACAGATGA

GGAATAGGAAATGTGATGCAAGCAGA[A/C]CAGGTTTACGTCATCTGTCGTTGCCCTATTCAA

rs4245739

A/C

GAGAAAAGACCACGAGACGG

TTTTCAAATAATGTGGTAAGTGCAC[G/T]

GAGAAAAGACCACGAGACGGAGACTGATTTGGCC[A/C]GTTCACTTACCACATTATTTGAAAA

rs2273953

A/G

TTCCTTCCTTCCTGCAGCGC[A/G]

AGAGCTCCAGAGGTGCTCAAA

TTCCTTCCTTCCTGCAGAGC[A/G]AAGAATTCACTGTGACTATTTGAGCACCTCTGGAGCTCT

rs319227

G/T

CCCTGCTCTAGAACATCGACCTA[A/C]

GCAGACCCAGGACTTGAATGC

CCCTGCTCTAGAACATCGACATA[A/C]GTGTACCCTGTATTGGCATTCAAGTCCTGGGTCTGC

rs2069347

C/T

CATATTTTAGTCCTCGGTATCTAACAAAG[T/C]

ATGACTTGTACATAAGAGCAACGATCT

CATATTTTAGTCCTCGGTATCTAACACAG[C/T]CTGAGATCGTTGCTCTTATGTACAAGTCAT

rs2426127

C/T

GGCTGACAGAATTCCTTTTTAGATGC

CCAAAGACCCTTAGCCCTAAATC[A/G]

GGCTGACAGAATTCCTTTTTAGATGCGAGTGCTCCG[C/T]GAGTTAGGGCTAAGGGTCTTTGG

rs1800054

C/G

ATTAAACATCTAGATCGGCATTCAGCTT[C/G]

TGCAAGGCATAATGATATATAGGAAGCAA

ATTAAACATCTAGATCGGCATTCAGATT[C/G]CATTGCTTCCTATATATCATTATGCCTTGCA

rs34330

C/T

CGTCGGGGTCTGTGTCTTTTG

AACAAAGCGCCCCTAAGC[A/G]

CGTCGGGGTCTGTGTCTTTTGCGGGGGGAGGGATCGAAATA[C/T]GCGTAGGGGCGCTTTGTT

rs1045485

C/G

TGCTCTCCAGCTGTGGTCTG

AGATTTGCTCTACTGTGCAGTAAT[C/G]

TGCTCTCCAGCTGTGGTCTGCTACAACTGGTAGGT[C/G]ATGACTGCACAGTAGAGCAAATCT

  1. Mismatches and allele-specific nucleotides are marked bold and underlined. The first 6 SNP assays were used for the determination of the optimal artificial mismatch location, assessment of the required DNA input concentration and assay specificity. The latter 6 SNP assays were only applied in the assay specificity and DNA input concentration experiments.