Table 3 Clinically relevant mutations and their corresponding oligonucleotide sequences.
From: Cost-effective and robust genotyping using double-mismatch allele-specific quantitative PCR
| Â | Â | allele | allele-specific primers | common primer |
|---|---|---|---|---|
rs113488022 | BRAF (V600E) | A/T | AATAGGTGATTTTGGTCTAGCTACAG[A/T] | GTAACTCAGCAGCATCTCAGGG |
rs113993960 | CF (deltaF508) | C/T | GCCTGGCACCATTAAAGAAAATATCAT[C/T] | GGCATGCTTTGATGACGCTTC |
rs6025 | Leiden mutation (FCTRV) | C/T | ACTTCAAGGACAAAATACCTGTATTCCT[C/T] | TCGCCTCTGGGCTAATAGGAC |
rs77375493 | JAK2 (V617F) | A/C | AGTTTTACTTACTCTCGTCTCCACAGA[A/C] | AGCAGCAAGTATGATGAGCAAGC |
COSM28057 | ALK (F1174L_A > G) | C/T | CCTCTCTGCTCTGCAGCAAA[C/T] | GGGTCTCTCGGAGGAAGGAC |
COSM28059 | ALK (F1174C) | A/G | CCTCTCTGCTCTGCAGCAAAT[G/T] | GGGTCTCTCGGAGGAAGGAC |
COSM28055 | ALK (F1174L_G > T) | A/C | CTCTCTGCTCTGCAGCAAATT[A/C] | GGGTCTCTCGGAGGAAGGAC |
COSM28056 | ALK (R1275Q) | C/T | CAGTCTTTACTCACCTGTAGATGTCT[C/T] | GCCAGAAACTGCCTCTTGACC |
rs17851045 | KRAS (codon 61) | A/T | CCCTCATTGCACTGTACTCCTC[A/T] | TTGTCCGTCATCTTTGGAGCAG |
COSM12429 | EGFR (L858R) | G/T | TGTCAAGATCACAGATTTTGGGC[G/T] | CTAGTGGGAAGGCAGCCTGG |
COSM6252 | EGFR (G719S) | A/G | ACTGAATTCAAAAAGATCAAAGTGCTG[A/G] | AGACCATGAGAGGCCCTGC |
COSM6253 | EGFR (G719C_G > T) | G/T | ACTGAATTCAAAAAGATCAAAGTGCTG[G/T] | AGACCATGAGAGGCCCTGC |
COSM18441 | EGFR (G719C_GG > TT) | A/C | GAACGCACCGGAGCC[A/C] | TGGAGCCTCTTACACCCAGTG |
COSM6239 | EGFR (G719A) | C/G | TGAATTCAAAAAGATCAAAGTGCTGG[C/G] | GCTCCCCACCAGACCATGAG |
COSM6240 | EGFR (T790M) | C/T | CCACCGTGCAGCTCATCA[C/T] | AGCAGGTACTGGGAGCCAAT |
