Figure 4 | Scientific Reports

Figure 4

From: Crystal structure and substrate interactions of an unusual fungal non-CBM carrying GH26 endo-β-mannanase from Yunnania penicillata

Figure 4

(A) Product profiles from guar gum hydrolysis by YpenMan26A and Wsp.Man26A. Aligned electropherograms of product profiles at 30% guar gum conversion (max conversion). Migration of oligosaccharides is given in dextran units (DE). A ladder was run containing: mannose (M1, 0.9 DE), mannobiose (M2, 1.87 DE), mannotriose (M3, 2.85 DE), and α-61-galactosyl-mannotriose (MMG, 3.81 DE). Migration of α-galactosyl-mannose (G, 2.10 DE), and α-62-61-di-galactosyl-mannotriose (MGG, 4.10 DE) was determined by von Freiesleben et al.26. (B) Initial reaction rates (U/µmole) by YpenMan26A and Wsp.Man26A on galactomannans. Data are from von Freiesleben et al.26. Hydrolyses were carried out at 37 °C, pH 5 on guar gum (light grey) and locust bean gum (dark grey). Values are given as mean values ± SD (n = 2). (C) The structure of YpenMan26A with MGG in the −4 to −2 subsites. The two differences in ligand binding amino acids between YpenMan26A and a superimposed homology model of Wsp.Man26A are highlighted in blue and orange, respectively.

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