Figure 5
From: The long non-coding RNA Meg3 is dispensable for hematopoietic stem cells
HSC stress response is only marginally affected in Meg3 mat KO HSCs. (A) Workflow depicting pIC-mediated Cre and interferon-mediated stress induction. (B) Flow cytometry-based cell cycle analysis of HSCs/MPPs. Relative percentage within each cell-cycle phase is shown. n = 4–11, mean + SD, two-way ANOVA. (C) HSCs, MPP1, MPP2, and MPP3/4 frequencies determined by flow cytometry analysis. Percentage of BM HSC-MPPs are represented. n = 4–18, one-way ANOVA. For all experiments: *p < 0.05; **p < 0.01; ***p < 0.001, ns: not significant. n indicates number of biological replicates; B-C: 1–4 independent experiments.
