Figure 7
From: Metabolic adaptation of adherent-invasive Escherichia coli to exposure to bile salts
Alternative metabolic pathways in AIEC strain LF82 are induced in the small intestine of mice. Sixteen mice were inoculated with AIEC strain LF82. Cholestyramine (2%), a bile acid sequestrant, was added or not to the drinking water. (A) The mRNA of AIEC strain LF82 was extracted from the ileum of mice, and qPCR was performed for representative genes of major metabolic pathways that we had identified in this study (ethanolamine and 1,2-propanediol utilization, citrate degradation and methyl-citrate pathway). (B) Ileal mucosal-associated LF82 bacteria from mice treated or not treated with cholestyramine (2%) were quantified. Values are means ± SEM. Statistical analysis was performed with a Mann-Whitney test; *p < 0.05.
